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cck 8 working solution (Beyotime)

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Beyotime cck 8 working solution

In vitro cytocompatibility assessment . <t>(a)</t> <t>CCK-8</t> assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Cck 8 Working Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 23302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cck+8+solution/Cell+Counting+Kit-8/pmc13092056-97-18-24
Average 99 stars, based on 23302 article reviews

cck 8 working solution - by Bioz Stars, 2026-09

99/100 stars

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1) Product Images from "Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation"

Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation

Journal: Bioactive Materials

doi: 10.1016/j.bioactmat.2026.04.003

In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Figure Legend Snippet: In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Techniques Used: In Vitro, CCK-8 Assay, Cell Culture, Staining, Migration

Incubation:

Article Title: Silencing Adamts2 attenuates fibroblast-mediated fibrosis and promotes axonal regeneration in an in vitro model.
Article Snippet: Fibrotic scars formed after central nervous system injury pose a strong barrier to axonal regeneration.. To attenuate the inhibitory effect of fibrotic scars, numerous pre-clinical studies have investigated strategies.. Fibroblasts are the main cells involved in the formation of fibrotic scars.

Article Title: A Brain‐Targeting Curcumin Analog Inhibits Glioblastoma Progression Through THBS1/TGF‐β1/PI3K–AKT Axis Modulation: Evidence From Experimental and Bioinformatic Analyses
Article Snippet: .. After 24–72 h of incubation, 10 μL of CCK‐8 solution (Super‐Enhanced Cell Counting Kit‐8, #C0048FT, Beyotime Biotechnology, Shanghai, China) was added to each well and incubated in the dark for 2 h in a CO2 incubator. ..

Article Title: Intestinal Lachnospiraceae bacterium -derived propionate inhibits the progression of clear cell renal cell carcinoma
Article Snippet: .. After 24 h of incubation, the medium was removed and replaced with 100 μL of fresh complete culture medium containing 10 μL of CCK-8 solution (Cell Counting Kit-8, Beyotime, China). .. The absorbance of each well was measured at 450 nm using a microplate reader (Thermo Scientific, Pittsburgh, PA, USA) to determine cell viability.

Article Title: Intestinal Lachnospiraceae bacterium-derived propionate inhibits the progression of clear cell renal cell carcinoma.
Article Snippet: .. After 24 h of incubation, the medium was removed and replaced with 100 μL of fresh complete culture medium containing 10 μL of CCK-8 solution (Cell Counting Kit-8, Beyotime, China). .. The absorbance of each well was measured at 450 nm using a microplate reader (Thermo Scientific, Pittsburgh, PA, USA) to determine cell viability.

Article Title: Cadmium exposure impaired the uterine decidualization through inducing the imbalance of mitochondrial fusion and fission.
Article Snippet: Cadmium (Cd), a widespread environmental pollutant, has toxic effects on spermatogenesis and sperm motility, but its role in uterine decidualization remains unknown.. This study revealed that Cd exposure during early pregnancy might impair the uterine decidualization along with the disordered proliferation and apoptosis of stromal cells, resulting in the reduction of mouse neonatal number and birth weight.. After disrupting the binding of Ca2+ and LNR-C, Cd exposure ligand-independently activated the NOTCH1 signaling and then restrained the expression of nuclear TAZ via RBPJ-targeting LATS.

Article Title: A Brain-Targeting Curcumin Analog Inhibits Glioblastoma Progression Through THBS1/TGF-β1/PI3K-AKT Axis Modulation: Evidence From Experimental and Bioinformatic Analyses.
Article Snippet: .. After 24–72 h of incubation, 10 μL of CCK- 8 solution (Super- Enhanced Cell Counting Kit- 8, #C0048FT, Beyotime Biotechnology, Shanghai, China) was added to each well and incubated in the dark for 2 h in a CO2 incubator. ..

CCK-8 Assay:

Article Title: Rutin ameliorates sevoflurane-induced neurotoxicity by inhibiting microglial synaptic phagocytosis through the complement pathway.
Article Snippet: .. BV2 cells were seeded in 96-well plates at a density of 1 × 104 cells/well and cultured with rutin for 12 h. Then, the cells were washed with PBS and cultured with a new medium containing 10 μL of CCK-8 solution (Enhanced Cell Counting Kit-8, Cat. no. C0043, Beyotime) in each well for 2 h at 37 °C. .. The absorbance was measured at 450 nm using a microplate reader (PerkinElmer EnSpire, Singapore), and the relative cell viability was calculated.

Article Title: Rutin ameliorates sevoflurane-induced neurotoxicity by inhibiting microglial synaptic phagocytosis through the complement pathway
Article Snippet: .. BV2 cells were seeded in 96-well plates at a density of 1 × 10 4 cells/well and cultured with rutin for 12 h. Then, the cells were washed with PBS and cultured with a new medium containing 10 μL of CCK-8 solution (Enhanced Cell Counting Kit-8, Cat. no. C0043, Beyotime) in each well for 2 h at 37 °C. .. The absorbance was measured at 450 nm using a microplate reader (PerkinElmer EnSpire, Singapore), and the relative cell viability was calculated.

Cell Culture:

Cell Counting:

Image Search Results

Journal: Bioactive Materials

Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation

doi: 10.1016/j.bioactmat.2026.04.003

Figure Lengend Snippet: In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: On days 1, 3, and 5 of culture, the existing culture medium was substituted with 100 μL of CCK-8 working solution (Cell Counting Kit-8, Beyotime Biotech, China), prepared by mixing fresh α-MEM and CCK-8 reagent at a volume ratio of 10:1.

Techniques: In Vitro, CCK-8 Assay, Cell Culture, Staining, Migration

SalB attenuated CORT-induced HT22 cell damage. (A) HT22 cells were exposed to 0–250 μM CORT for 24 h, and CCK-8 assessed cell viability. (B) HT22 cells were treated with 2.5–40 μM SalB for 24 h, and CCK-8 assessed cell viability. (C) HT22 cells were pretreated with 10, 15 and 20 μM SalB for 1 h, and then stimulated with 200 μM CORT for 24 h. CCK-8 assessed cell viability. SalB significantly increased it. (D) The LDH level of the cells was detected by the kit, which was significantly reduced after SalB intervention. (E, F) Flow cytometry detected apoptosis level, which was significantly reduced after SalB intervention. (G–I) ELISA detected inflammatory factor content. SalB markedly declined TNF-α, IL-1β and IL-6 contents. (J, K) The intracellular ROS level was assessed using flow cytometry, which was markedly lessened after SalB intervention. (L–N) Oxidative stress indexes were detected by kits. SalB significantly increased GSH-Px and SOD activities and decreased MDA contents. n = 3, ** p < 0.01, *** p < 0.001 vs. 0/Control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CORT group.

Journal: Frontiers in Neurology

Article Title: Salvianolic acid B alleviates depression-like behaviors by reducing neuronal injury and promoting neurogenesis in a manner associated with JAK-STAT signaling pathway inhibition

doi: 10.3389/fneur.2026.1817261

Figure Lengend Snippet: SalB attenuated CORT-induced HT22 cell damage. (A) HT22 cells were exposed to 0–250 μM CORT for 24 h, and CCK-8 assessed cell viability. (B) HT22 cells were treated with 2.5–40 μM SalB for 24 h, and CCK-8 assessed cell viability. (C) HT22 cells were pretreated with 10, 15 and 20 μM SalB for 1 h, and then stimulated with 200 μM CORT for 24 h. CCK-8 assessed cell viability. SalB significantly increased it. (D) The LDH level of the cells was detected by the kit, which was significantly reduced after SalB intervention. (E, F) Flow cytometry detected apoptosis level, which was significantly reduced after SalB intervention. (G–I) ELISA detected inflammatory factor content. SalB markedly declined TNF-α, IL-1β and IL-6 contents. (J, K) The intracellular ROS level was assessed using flow cytometry, which was markedly lessened after SalB intervention. (L–N) Oxidative stress indexes were detected by kits. SalB significantly increased GSH-Px and SOD activities and decreased MDA contents. n = 3, ** p < 0.01, *** p < 0.001 vs. 0/Control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CORT group.

Article Snippet: Subsequently, 10 μl of cell counting kit (CCK)-8 solution (G4103, Servicebio, Wuhan, China) was added and incubated for 2 h. The optical density (OD) at 450 nm was detected by a microplate reader (1410101, ThermoFisher Scientific, Waltham, MA, USA), and the cell viability was calculated to determine the optimal administration conditions.

Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control

SalB attenuated CORT-induced HT-22 cell injury by JAK-STAT signaling. (A) CCK-8 detected cell viability. RO8191 significantly reduced cell viability. (B) The LDH level of cells was detected by the kit, which was significantly increased after RO8191 intervention. (C, D) Flow cytometry detected apoptosis level, which was significantly increased after RO8191 intervention. (E–G) ELISA detected inflammatory factor content. RO8191 markedly enhanced TNF-α, IL-1β and IL-6 contents. (H–J) Oxidative stress index was detected by kit. RO8191 significantly reduced GSH-Px and SOD activities and increased MDA contents. (K, L) The intracellular ROS level was detected by flow cytometry, which was significantly increased after RO8191 intervention. n = 3, *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. CORT group; & p < 0.05, && p < 0.01 vs. CORT+20 μM SalB group.

Journal: Frontiers in Neurology

Article Title: Salvianolic acid B alleviates depression-like behaviors by reducing neuronal injury and promoting neurogenesis in a manner associated with JAK-STAT signaling pathway inhibition

doi: 10.3389/fneur.2026.1817261

Figure Lengend Snippet: SalB attenuated CORT-induced HT-22 cell injury by JAK-STAT signaling. (A) CCK-8 detected cell viability. RO8191 significantly reduced cell viability. (B) The LDH level of cells was detected by the kit, which was significantly increased after RO8191 intervention. (C, D) Flow cytometry detected apoptosis level, which was significantly increased after RO8191 intervention. (E–G) ELISA detected inflammatory factor content. RO8191 markedly enhanced TNF-α, IL-1β and IL-6 contents. (H–J) Oxidative stress index was detected by kit. RO8191 significantly reduced GSH-Px and SOD activities and increased MDA contents. (K, L) The intracellular ROS level was detected by flow cytometry, which was significantly increased after RO8191 intervention. n = 3, *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. CORT group; & p < 0.05, && p < 0.01 vs. CORT+20 μM SalB group.

Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control

Effects of miR-32-5p on the proliferative capacities, migratory abilities and apoptotic rate of UCEC cells. (A) The expression of miR-32-5p in HEC-1-A and Ishikawa cells were then determined using quantitative reverse transcription-PCR. ***P<0.001 vs. HEC-1-A cells. (B) The expression of miR-32-5p in HEC-1-A cells after transfection of miR-32-5p inhibitor or in Ishikawa cells after transfection of miR-32-5p mimic. (C) The viability of UCEC cell lines were measured using the CCK-8 assay. (D) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (E) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (F) The migratory potential of UCEC cell lines were determined via Transwell migration assay. (Scale bar, 100 µm). (G) Flow cytometry was used to detect apoptosis. * P<0.05, **P<0.01, ***P<0.001 vs. miR-32-5p inhibitor-NC or miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; EdU, 5-ethynyl-2′-deoxyuridine.

Journal: Oncology Letters

Article Title: Exosomal-miR-32-5p directly targets FOXN2 to regulate the proliferation, migration and apoptosis of uterine corpus endometrial carcinoma via the PI3K/AKT/BCL-2 pathway

doi: 10.3892/ol.2026.15574

Figure Lengend Snippet: Effects of miR-32-5p on the proliferative capacities, migratory abilities and apoptotic rate of UCEC cells. (A) The expression of miR-32-5p in HEC-1-A and Ishikawa cells were then determined using quantitative reverse transcription-PCR. ***P<0.001 vs. HEC-1-A cells. (B) The expression of miR-32-5p in HEC-1-A cells after transfection of miR-32-5p inhibitor or in Ishikawa cells after transfection of miR-32-5p mimic. (C) The viability of UCEC cell lines were measured using the CCK-8 assay. (D) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (E) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (F) The migratory potential of UCEC cell lines were determined via Transwell migration assay. (Scale bar, 100 µm). (G) Flow cytometry was used to detect apoptosis. * P<0.05, **P<0.01, ***P<0.001 vs. miR-32-5p inhibitor-NC or miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; EdU, 5-ethynyl-2′-deoxyuridine.

Article Snippet: HEC-1-A or Ishikawa cells (3×10 3 cells/ml) were grown in 96-well plates for 0, 24, 48 and 72 h before adding the CCK-8 solution (15 μl; Dojindo Laboratories, Inc.).

Techniques: Expressing, Reverse Transcription, Transfection, CCK-8 Assay, Colony Assay, Proliferation Assay, Transwell Migration Assay, Flow Cytometry, Negative Control

Exo-miR-32-5p regulates the proliferation, migration and apoptosis of UCEC cells through regulating FOXN2 expression and PI3K/AKT/Bcl-2 pathway. The impact of Exo-miR-32-5p on the proliferation, migration and apoptosis of UCEC cells were examined utilizing a co-culture model. (A) Exo-miR-32-5p expression in HEC-1-A cells transfected with miR-32-5p-inhibitor or in Ishikawa cells transfected with miR-32-5p-mimic was determined through qRT-PCR. (B) The viability of UCEC cell lines were measured using the CCK-8 assay. (C) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (D) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (E) The migratory potential of UCEC cell lines were determined via the Transwell migration assay. (F) Flow cytometry was used to detect apoptosis. (G) The mRNA expression of AKT, PI3K, Bcl-2 and FOXN2 was detected by qRT-PCR. (H) The protein levels of AKT, PI3K, p-PI3K, Bcl-2, FOXN2 and the ratio of p-AKT/AKT were measured by western blotting. **P<0.01, ***P<0.001 vs. Exo-miR-32-5p inhibitor-NC or Exo-miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; NS, not significant; WT, wild-type; MUT, mutant; qRT-PCR, quantitative reverse transcription-PCR; p-, phosphorylated; FOXN2, Forkhead Box N2; EdU, 5-ethynyl-2′-deoxyuridine.

Journal: Oncology Letters

Article Title: Exosomal-miR-32-5p directly targets FOXN2 to regulate the proliferation, migration and apoptosis of uterine corpus endometrial carcinoma via the PI3K/AKT/BCL-2 pathway

doi: 10.3892/ol.2026.15574

Figure Lengend Snippet: Exo-miR-32-5p regulates the proliferation, migration and apoptosis of UCEC cells through regulating FOXN2 expression and PI3K/AKT/Bcl-2 pathway. The impact of Exo-miR-32-5p on the proliferation, migration and apoptosis of UCEC cells were examined utilizing a co-culture model. (A) Exo-miR-32-5p expression in HEC-1-A cells transfected with miR-32-5p-inhibitor or in Ishikawa cells transfected with miR-32-5p-mimic was determined through qRT-PCR. (B) The viability of UCEC cell lines were measured using the CCK-8 assay. (C) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (D) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (E) The migratory potential of UCEC cell lines were determined via the Transwell migration assay. (F) Flow cytometry was used to detect apoptosis. (G) The mRNA expression of AKT, PI3K, Bcl-2 and FOXN2 was detected by qRT-PCR. (H) The protein levels of AKT, PI3K, p-PI3K, Bcl-2, FOXN2 and the ratio of p-AKT/AKT were measured by western blotting. **P<0.01, ***P<0.001 vs. Exo-miR-32-5p inhibitor-NC or Exo-miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; NS, not significant; WT, wild-type; MUT, mutant; qRT-PCR, quantitative reverse transcription-PCR; p-, phosphorylated; FOXN2, Forkhead Box N2; EdU, 5-ethynyl-2′-deoxyuridine.

Article Snippet: HEC-1-A or Ishikawa cells (3×10 3 cells/ml) were grown in 96-well plates for 0, 24, 48 and 72 h before adding the CCK-8 solution (15 μl; Dojindo Laboratories, Inc.).

Techniques: Migration, Expressing, Co-Culture Assay, Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Proliferation Assay, Transwell Migration Assay, Flow Cytometry, Western Blot, Negative Control, Mutagenesis, Reverse Transcription

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cck 8 working solution (Beyotime)

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1) Product Images from "Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation"

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